Bacterio-opsin mutants of Halobacterium halobium

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Bacterio-opsin mutants of Halobacterium halobium.

The bacterio-opsin (bop) gene of Halobacterium halobium R1 has been cloned with about 40 kilobases of flanking genomic sequence. The 40-kilobase segment is derived from the (G+C)-rich fraction of the chromosome and is not homologous to the major (pHH1) or minor endogenous covalently closed circular DNA species of H. halobium. A 5.1-kilobase Pst I fragment containing the bop gene was subcloned i...

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Bacterio-opsin mRNA in wild-type and bacterio-opsin-deficient Halobacterium halobium strains.

We have examined transcripts corresponding to the Halobacterium halobium bacterio-opsin (BO) gene in wild-type and in BO-deficient mutant strains containing the insertion elements ISH1 or ISH2 in the BO gene. BO mRNA from the wild-type strain was purified by hybrid selection using single-stranded cDNA. Labeling by vaccinia virus capping enzyme and [alpha-(32)P]GTP showed that it contains the 5'...

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Characterization of insertions affecting the expression of the bacterio-opsin gene in Halobacterium halobium.

We have determined the sequence of the inverted repeats and duplicated target DNA of the halobacterial insertion elements ISH2 (520 bp), ISH23 (900 bp) and ISH24 (3000 bp) associated with bacterio-opsin (bop) mutants. ISH2 has a perfect 19 bp inverted repeat (3,5), while both ISH23 and ISH24 have imperfect inverted repeats of 29 bp and 14 bp respectively. ISH23 was shown to be highly homologous...

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Genetic variability in Halobacterium halobium.

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Integration of photosensory signals in Halobacterium halobium.

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 1983

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.80.5.1416